Muscle α-tocopherol level did not affect muscle CD or TBARS. 05) following RSET but were not affected by dietary vitamin E level. Serum CK and AST activities increased ( P <.
05) at 30 and 90 d in horses receiving the 300 diet than in horses receiving the BASAL and 80 diets. Serum and muscle α-tocopherol concentrations were higher ( P <. 06, respectively) during the 90-d treatment period but did not change in horses receiving the 300 diet. Serum α-tocopherol concentrations of horses receiving the BASAL and 80 diets decreased ( P <.
The following were measured before and after the RSET: α-tocopherol, thiobarbituric acid reactive substances (TBARS), conjugated diene (CD) concentrations of the middle gluteal muscle, and serum creatine kinase (CK) and aspartate aminotransferase (AST) activities. All horses performed a repeated submaximal exercise test (RSET) at the end of the 90-d period. During the 90-d treatment period, horses were exercised 5 d/wk in addition, serum and middle gluteal muscle α-tocopherol concentrations were measured at 0, 30, and 90 d. The BASAL diet contained less than 44 IU of vitamin E/kg DM, but it was adequate in all other nutrients. Nineteen horses were blocked by sex and then assigned to one of three diets: no supplemental vitamin E (BASAL), BASAL plus 80 IU of supplemental vitamin E/kg DM (80), or BASAL plus 300 IU of supplemental vitamin E/kg DM (300).
The effect of vitamin E intake on indicators of muscle integrity was studied in exercised horses.
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